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Mouse anti-Human Ig (Chain kappa), (Light Chain) Antibody (PE)

FACS Monoclonal A8B5 IgG1 PE
Catalog No. ABIN238382

Quick Overview for Mouse anti-Human Ig (Chain kappa), (Light Chain) Antibody (PE) (ABIN238382)

Target

Ig

Clonality

  • 123
  • 95
Monoclonal

Conjugate

  • 54
  • 25
  • 23
  • 22
  • 18
  • 14
  • 12
  • 10
  • 5
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
PE

Application

  • 173
  • 138
  • 101
  • 69
  • 57
  • 26
  • 22
  • 12
  • 12
  • 7
  • 7
  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
Flow Cytometry (FACS)

Clone

A8B5

Isotype

IgG1
  • Binding Specificity

    • 80
    • 78
    • 63
    • 38
    • 4
    • 2
    • 1
    Chain kappa, Light Chain

    Reactivity

    • 118
    • 63
    • 20
    • 18
    • 9
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human

    Host

    • 102
    • 77
    • 39
    • 18
    • 3
    • 1
    • 1
    Mouse

    Purification

    Size-exclusion chromatography.
  • Application Notes

    Suitable for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL ofwhole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Buffer

    PBS containing 15 mM sodium azide as preservative and 0.2 % (w/v) high-grade BSA (Protease free) as stabilizer.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C

    Storage Comment

    Store the antibody in the dark at 2-8 °C. Do Not Freeze! Avoid prolonged exposure to light.
    Shelf life: One year from despatch.

    Expiry Date

    12 months
  • Target

    Ig

    Alternative Name

    Immunoglobulin

    Background

    Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
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